Forth Dimension Displays’ LCOS-based Fast Switching Binary Modulators enable new Ultra-Fast, High-Resolution Microscopes
Forth Dimension Displays (ForthDD) and Intelligent Imaging Innovations (3i), a Colorado-based manufacturer of fluorescence microscope systems for biomedical research, expanded their collaboration with the launch of 3i’s Cleared Tissue LightSheet (CTLS) product. CTLS can image whole organs at high speed by illuminating them with structured light using ForthDD’s proprietary high-resolution, fast Spatial Light Modulators (SLMs).
Similar to other fluorescence microscope techniques, CTLS uses laser light to stimulate fluorescence emission from genetically-labelled – or stained – tissue to form an image of the specimen. The Cleared Tissue LightSheet creates a finely focused sheet of light for optical sectioning, then uses ForthDD’s SLM to rapidly shift the thinnest portion of the sheet in synchronization with the microscope and camera resulting in ultra-fast and high-resolution imaging of large specimens. Piezoelectric stages move the specimen in x, y, and z directions with sub-micron precision. The emission from the fluorescent markers in the specimen is recorded by a camera and 3i SlideBook software automatically and precisely reconstructs the biological structure in 3D for further analysis. Hundreds to thousands of images are required to create a 3D image of the tissue sample.
3i also integrates ForthDD’s SLMs into their live cell imaging system Lattice LightSheet (LLS). The SLM is used to create an ultra-thin lattice light sheet which can be quickly adjusted for biological conditions on-the-fly due to the flexibility and speed of ForthDD’s SLM. LLS enables spatiotemporal super-resolution imaging of dynamic cellular processes with ultra-fast capture speeds and high resolution, while limiting laser light damage to the cells. LLS can also be used to perform structured illumination microscopy (SIM), a super-resolution technique that improves the spatial resolution to the low 100’s of nanometers.
“Spatial light modulators provide outstanding value, increasing the speed and resolution of research microscopes,” Brian Bodensteiner, Vice President Development of 3i said, “ForthDD’s SLM technology is designed to allow for the flexibility, performance, and hardware-timing demanded by advanced light microscopy applications.”
Greg Truman, CEO of ForthDD said, “The development of super-resolution techniques has been a remarkable achievement and ForthDD is very pleased that our fast binary SLMs are a key enabler for microscope developers and biologists around the globe. The new microscopes are now being used in the life sciences industry to enhance the understanding of the biological structures. ForthDD is committed to supporting its customers to create their most advanced products.”